Microscopy

Grossing

Macroscope Z12APO Leica

Manager : Béatrice Hechler

Confocal microscopy

Confocal microscope LAMBDA DG-4 INTELLIGENT IMAGING I

Inverted confocal microscope Leica TCS SP5 AOBS

including 2 types of conventional and fast scanner (Resonance). It is equipped with laser lines 458, 476, 488, 496, 514, 561, 633 and diode 405. 4 immersion objectives are present (x20 / 0.7- x40 / 1.25- x63 / 1.4 – x100 / 1,3), a thermostatic chamber and a motorized stage.

Capacities :

  • Live or fixed samples
  • Control of temperature, humidity and CO2
  • Resonance scanner
  • 3D reconstruction, real time

Manager : Pierre Mangin

Fluorescence microscopy

Inverted fluorescence microscope Leica DMI/IRB

Allows sample observation in transmitted light (including differential interference contrast technique) as well as in fluorescence. This microscope has various air objectives (x10 / 0.25 – x40 / 0.55 – x63 / 0.7) and immersion objectives (x40 / 1.25 – x63 / 1.4). Image acquisition is performed by a CoolSNAP cf2 camera via the Metamorph software.

Inverted fluorescence microscope Leica DMI4000

Allows sample observation in transmitted light (including differential interference contrast technique) as well as in fluorescence. It is equipped with 4 fluorescence filters (DAPI, FITC, TRITC and Cy5) and an EL6000 excitation source. This microscope has various lenses (x20-x40-x63-x100) and a CoolSNAP HQ2 camera. Image acquisition is performed by the Metamorph software.

Microscope Leica DMR

Allows sample observation in transmitted light as well as in fluorescence.

Manager : Pierre Mangin

Electron microscopy

The electron microscopy platform is dedicated to the ultrastructural study of blood platelets and their precursors, the megakaryocytes. It is equipped with a Scanning Electron Microscope (SEM) to observe the surface of samples and changes in the shape of blood cells, and a Transmission Electron Microscope (TEM) to observe sample cuts.

In 2011, the laboratory acquired a new equipment, the Dual Beam electron microscope allowing 3D reconstruction of whole cells (100 micrometers of tissues or cells), an objective that remained, until now, impossible to achieve in many years. such proportions and on this scale.

 

Skills

  • Inclusion of Epon resin biological samples for ultrastructural studies
  • Immunolabeling on cryosections (according to the Tokuyasu protocol) to locate proteins at the ultrastructural scale
  • Negative staining to view platelets in toto (whole mount)
  • Sample Preparation for Scanning Electron Microscopy and Dual Beam. Image processing uses the Amira software for 3D reconstructions.
  • Correlative microscopy to observe the same cell in light microscopy and electron microscopy.

Equipments

Manager : Anita Eckly-Michel