Following erosion or rupture of an atherosclerotic plaque, platelets accumulate at the site of injury and form aggregates (thrombi) that can clog the vessel, resulting in serious ischemic conditions such as heart attack. myocardium or stroke. Platelet activation, which increases adhesive properties, plays a key role in their aggregation. Activated platelets have been described to interact with other circulating cells, including leukocytes, allowing their recruitment to a thrombus. The role of platelet-leukocyte interaction in thrombus formation is unclear. The aim of this work will be to study the platelet-leukocyte interaction in pathological flow conditions as found at the level of an atherosclerotic plaque presenting a severe stenosis. For this, microfluidic chambers mimicking arterial stenosis of 90% will be developed. Anticoagulated blood will be infused onto atherosclerotic plaque proteins and platelet-leukocyte interactions will be studied in real time by video microscopy. In parallel, an in vivo model will be developed in which these interactions will be observed in real time by video microscopy, after lesion of the vascular wall of a carotid of a mouse with different degrees of stenosis.

Role of pathological flow on platelet-leukocyte interactions

Technologies acquired at the end of the M2 internship:

  • Brightfield microscopy
  • Epifluorescence
  • Confocal microscopy

Manager : Christian Gachet

Team “Biology and pharmacology of blood platelets” – Christian Gachet

Internship manager : Pierre Mangin (CR1 INSERM)

Candidate recruited in the specialty :