Biacore 3000

Biacore technology is used to measure molecular interactions in real time without labeling one of the two interactants for different classes of molecules.

The principle of signal detection is based on surface plasmon resonance that reflects mass variations on the surface of a chip on which the ligand is covalently immobilized or not. The other interactant (the analyte) is injected by a microfluidic system into a continuous stream of buffer on the surface of the sensor chip.

Real-time monitoring makes it possible to determine the kinetic constants of association and dissociation, and to deduce the value of the affinity constant. It allows the characterization of molecular interactions in a purified or complex medium

Coupled with mass spectrometry, Biacore allows the molecular characterization of analytes.

 

Manager : Catherine Ravanat